The US Pharmacopeia (USP) is a standard source for many pharmaceutical methods that specifies columns by packing material rather than by manufacturer. Listed below are the recommended Agilent Technologies HPLC columns suitable for most LC methods listed with the USP.
| USP Method |
USP Packing Materials |
Column |
Particle Size(pm) |
| L1 |
Octadecyl Silane chemically bonded to porous silica or
ceramic micro-particles, 1.5 to 10 pm in diameter |
| Poroshell 120 EC- C18 |
| Poroshell 120 SB- C18 |
| ZORBAX Eclipse Plus C18 |
| ZORBAX Eclipse XDB-C18 |
| ZORBAX SB-C18 |
| ZORBAX Rx-C18 |
| ZORBAX Extend-C18 |
| ZORBAX ODS |
| ZORBAX ODS classic |
| Pursuit XRs C18 |
| Pursuit C18 |
| Polaris C18-A |
| Polaris C18-Ether |
| Sep Tech ST60 C18 |
| Sep Tech ST150 C18 |
|
| 2.7 |
| 2.7 |
| 1.8,3.5,5 |
| 1.8,3.5,5,7 |
| 1.8,3.5,5,7 |
| 3.5,5 |
| 1.8,3.5,5,7 |
| 3.5,5,7 |
| 5 |
| 3,5,10 |
| 3,5,10 |
| 3,5,10 |
| 3,5 |
| 10 |
| 10 |
|
| L2 |
Octadecyl Silane chemically bonded to porous silica gel
of a controlled surface porosity that has been bonded
to a solid spherical core, 30 to 50 pm in diameter |
N/A |
|
| L3 |
Porous silica particles, 5 to 10
pm in diameter |
| ZORBAX SIL |
| ZORBAX Rx-Sil |
| Pursuit XRs Si |
| Polaris Si-A |
| MicroSpher Si |
| Microsorb 100 Si |
|
|
| L4 |
Silica get of controlled surface porosity bonded to a solid spherical core, 30 to 50 pm in diameter |
|
|
| L5 |
Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50 pm in diameter |
N/A |
|
| L6 |
Strong cation-exchange
packing: sulfonated fluorocarbon polymer coated on a solid spherical core, 30 to 50 pm in diameter |
N/A |
|
| L7 |
Octyl Silane chemically bonded to totally porous
microsilica particles, 1.5 to 10 pm in diameter |
| Poroshell 120 EC-C8 |
| ZORBAX Eclipse Plus C8 |
| ZORBAX Eclipse XDB-C8 |
| ZORBAX SB-C8 |
| ZORBAX RX-C8 |
| ZORBAX C8 |
| Pursuit XRs C8 |
| Pursuit C8 |
| Polaris C8-A |
| Polaris C8-Ether |
| Microsorb 100 C8 |
|
| 2.7 |
| 1.8,3.5,5 |
| 1.8,3.5,5,7 |
| 1.8,3.5,5,7 |
| 1.8,3.5,5,7 |
| 5 |
| 3,5,10 |
| 3,5,10 |
| 3,5 |
| 3,5 |
| 5 |
|
| L8 |
An essentially monomolecular layer of
aminopropylsilane chemically bonded to totally porous silica gel support, 10 pm in diameter |
| ZORBAX NH2 |
| Polaris NH2 |
| Microsorb 100 Amino |
|
|
| L9 |
10 pm irregular, totally porous silica gel having a chemically bonded, strongly acidic cation
exchange coating |
ZORBAX SCX |
5 spherical |
| L10 |
Nitrile groups chemically
bonded to porous silica particles, 3 to 10 pm in diameter |
| ZORBAX CN |
| ZORBAX SB-CN |
| ZORBAX Eclipse XDB-CN |
| Microsorb 100 Cyano |
|
|
| L13 |
Trimethylsilane chemically
bonded to porous silica particles, 3 to 10 pm in diameter |
ZORBAX TMS |
5 |
| L14 |
Silica gel 10 pm in diameter
with a chemically bonded, strongly basic quaternary ammonium anion exchange coating |
|
|
| L15 |
Heryl silane chemically bonded to totally porous silica particles, 3 to 10 pm in diameter |
MetaSil C6 |
|
| L16 |
Dimethyl silane chemically bonded to totally porous
silica particles, 3 to 10 pm in diameter |
N/A |
|
| L17 |
Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 pm in diameter |
Hi-Plex H |
8 |
| |
Amino and cyano groups chemically bounded to porous silica particles, 5 to 10 pm in diameter |
N/A |
|
| L19 |
Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, 9 pm in diameter |
| Hi-Plex Ca |
| Hi-Plex Ca (Duo) |
|
|
| L20 |
Dihydroxypropane groups chemically bonded to porous silica particles, 3 to 10 pm in diameter |
LiChrospher Diol |
5 |
| L21 |
A rigid, spherical styrene divinylbenzene copolymer,
5 to 10 pm in diameter |
| PLgel |
| PLRP-S 100A |
| PLRP-S 300A |
| PLRP-S 1000A |
|
| 3,5,10,20 |
| 3,5,8 |
| 3,5,8 |
| 5,8 |
|
| L22 |
A cation exchange resign made of porous polystyrene
gel wioth sulfonic acid groups, about 10 pm in size |
Hi-Plex H |
8 |
| L23 |
An ion exchange resign made of porous
polymethacrylate or polyacrylate gel with quaternary ammonia groups, about 10 pm in size. |
N/A |
|
| L24 |
A semi-rigid hydrophilic gel consisting of vinyl
polymers with numerous hydroxyl group on the matrix surface, 32 to 63 pm in diameter |
N/A |
|
| L25 |
Packing having the capacity to separate compounds with
a MW range from 1,000 to 5,000 da (as determined
by the polyethylene oxide), applied to neutral, ionic and cationic water-soluble polymers |
PL aquagel-OH |
5,8 |
| L26 |
Butyl silane chemically bounded to porous silica
particles, 5 to 10 pm in diameter |
MicroSorb C4 |
5 |
| L27 |
Porous silica particles, 30 to 50 pm in diameter |
Bondesil Silica |
|
| L28 |
A multifunctional support, which consists of a high
purify, 100 A, spherical silica substrate that has been bonded with anionic (amine) functionality in addition to conventional reversed-phase C8 functionality |
N/A |
|
| L29 |
Gamma alumina, reversed phase, low carbon percentage by weight, alumina based polybutadiene spherical
particles, 5 pm diameter with a pore diameter of 80A |
N/A |
|
| L30 |
Ethyl silane chemically bonded to a totally porous
silica particles, 3 to 10 pm in diameter |
N/A |
|
| L31 |
A strong anion-exchange resin-quaternary amine
bonded on latex particles attached to a core of 8.5 pm macroporous particles having a pore size of 2000A
and consisting of ethylvinylbenzene cross-linked
with 55% divinylbenzene |
N/A |
|
| L32 |
A chiral ligand-exchange packing L- praline copper
complex covalently bonded to irregularly shaped silica particles, 5 to 10 pm in diameter |
N/A |
|
| L33 |
Packing having the capacity to separate proteins
by molecular size over a range of 4,000 to 400,000 da.
It is spherical, silica-based, and processed to provide pH stability |
| ZORBAX GF-250 |
| Bio SEC-3 |
| Bio SEC-5 |
|
|
| L34 |
Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, 9 pm in diameter |
Hi-Plex Pb |
8 |
| L36 |
A zirconium stabilized Spherical Silica Packing with a hydrophilic (diol – Type) molecular monolayer bonded phase |
| ZORBAX GF – 250 |
| ZORBAX GF – 450 |
|
|
| L37 |
Polymethcrylate get packing having the capacity to separate proteins by molecular size over a range of
2,000 to 4,000 da MW |
N/A |
|
| L39 |
Hydrophilic Polyhydroxymethacrylate Porous spherical resin |
N/A |
|
| L40 |
Cellulose tris-3.5, dimethylphenlycarbamate coated
porous silica particles, 5 to 20 pm in diameter |
N/A |
|
| L41 |
Immobilized alpha – acid glyci-protein on spherical
silica particles, 5pm in diameter |
N/A |
|
| L42 |
Octylsilane and octadecylsilane groups chemically bonded to porous silica particles |
N/A |
|
| L43 |
Pentafluorophenyl groups chemically bonded to silica particles 5 to 10 pm in diameter |
Pursuit PFP |
3.5 |
| L44 |
A Multifunctional support, which consists of a high
purity, 60A spherical silica substrate, that has been
bonded with a cationic exchanger, sulfonic acid
functionality in addition to a conventional
reversed phase C8 functionality |
N/A |
|
| L45 |
Beta cyclodextrin bonded to porous silica particles,
5 to 10 pm in diameter |
ChiraDex Chiral |
5 |
| L46 |
Polystyrene/divinylbenzene substrate agglomerated
with quaternary amine functionalized latex beads,
10 pm in diameter |
N/A |
|
| L47 |
High capacity anion exchange microporous substrate,
fully functionalized with a trimethyl-amine group,
8 pm in diameter |
N/A |
|
| L48 |
Sulfonated, cross-linked polystyrene with an outer layer
of submicron, porous, anion-exchange microbeads,
15 pm in diameter |
N/A |
|
| L49 |
Amylose tris-3,5-dimethylphenyl-carabamate-coated, porous, spherical, silica particles, 5 to 10
pm in diameter |
N/A |
|
| L50 |
A strong cation exchange resin made of porous silica
with sulfopropyl groups, 5 to 10 pm in diameter |
ZORBAX 300SCX |
5 |
| L51 |
A reversed-phase packing made by coating a thin
layer of polybutadiene on to spherical porous zirconia particles, 3 to 10 pm in diameter |
N/A |
|
| L52 |
Multifunction resin with reversed-phase retention and strong anion-exchange functionalities. The resin consists
of ethylvinyl-benzene, 55% cross-linked with divinyl-benzene copolymer, 3 to 15 pm in diameter, and a
surface area of not less than 350m2/g, substrate is
coated with quaternary ammonium functionalized latex particles consisting of styrene cross-linked with divinylbenzene. |
N/A |
|
| L53 |
An anion-exchange resin consisting of rigid, spherical styrene-divinylbenzene copolymer with trimethylammonium group at a loading of about 2
meq per g, 3 to 29 pm in diameter |
Bio SAX |
3,5,10 |
| L54 |
Strong cation exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, 7 to 11 pm in diameter |
N/A |
|
| L55 |
Weak cation exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 3 to 15 pm diameter. Substrate is surface grafted with carboxylic acid and/or phosphoric acid functionalized monomers. Capacity not less than 500 peq/column |
N/A |
|
| L56 |
Propyl silane chemically bonded to totally porous silica particles, 3 to 10 pm in diameter |
SB-C3 |
3,5 |
| L57 |
A chiral-recognition protein, ovomucoid, chemically
bonded to silica particles, about 5 pm in diameter,
with a pore size of 120 angstroms |
Ultron ES-OVM |
5 |
| L58 |
Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, about 6 to 30 pm in diameter |
| Hi-Plex Na |
| Hi-Plex Na (Octo) |
|
|
| L59 |
Packing having the capacity to separate proteins by molecular weight over the range of 5 to 7000 kDa. It is spherical (5-10 pm), silica-based, and processed to
provide hydrophilic characteristics and pH stability |
N/A |
|
| L60 |
Spherical, porous silica gel, 10 pm or less in diameter,
the surface of which has been covalently modified with alkyl amide groups and endcapped |
| Bonus-RP |
| Polaris Amide-C18 |
|
|
